SubtiBank SubtiBank
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2020-04-01 14:37:452025-04-04 16:23:12

Biological materials

Expression vectors

pGP2797: expression of ''darA'' (native RBS) by [SW|pBQ200] in ''B. subtilis'', available in [SW|Jörg Stülke]'s lab

pGP3002: expression of ''darA'' (''gapA'' RBS) by [SW|pBQ200] in ''B. subtilis'', available in [SW|Jörg Stülke]'s lab

pGP2601: IPTG inducible expression, purification in ''E. coli'' with N-terminal His-tag, in [SW|pWH844], available in [SW|Jörg Stülke]'s lab [Pubmed|25433025]

pGP1690: IPTG inducible expression, purification in ''E. coli'' with N-terminal cleavable His-tag, in [SW|pGP570], available in [SW|Jörg Stülke]'s lab

pGP2624: IPTG inducible expression, purification in ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172], available in [SW|Jörg Stülke]'s lab

pGP2602: expression of Strep-''darA'' by [SW|pGP380] in ''B. subtilis'' suitable for [SW|SPINE], available in [SW|Jörg Stülke]'s lab

pGP2603: expression of ''darA''-Strep by [SW|pGP382] in ''B. subtilis'' suitable for [SW|SPINE], available in [SW|Jörg Stülke]'s lab

GP2010: expression of ''darA''-Strep in ''B. subtilis'' (chromosomal), suitable for [SW|SPINE], available in [SW|Jörg Stülke]'s lab

pGP2797: expression of ''darA'' (native RBS) by [SW|pBQ200] in ''B. subtilis'', available in [SW|Jörg Stülke]'s lab

pGP3002: expression of ''darA'' (''gapA'' RBS) by [SW|pBQ200] in ''B. subtilis'', available in [SW|Jörg Stülke]'s lab

pGP2601: IPTG inducible expression, purification in ''E. coli'' with N-terminal His-tag, in [SW|pWH844], available in [SW|Jörg Stülke]'s lab [Pubmed|25433025]

pGP1690: IPTG inducible expression, purification in ''E. coli'' with N-terminal cleavable His-tag, in [SW|pGP570], available in [SW|Jörg Stülke]'s lab

pGP2624: IPTG inducible expression, purification in ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172], available in [SW|Jörg Stülke]'s lab

pGP2602: expression of Strep-''darA'' by [SW|pGP380] in ''B. subtilis'' suitable for [SW|SPINE], available in [SW|Jörg Stülke]'s lab

pGP2603: expression of ''darA''-Strep by [SW|pGP382] in ''B. subtilis'' suitable for [SW|SPINE], available in [SW|Jörg Stülke]'s lab

pGP2847: expression of His6-''darA'' (''gapA'' RBS) by [SW|pBQ200] in ''B. subtilis'', available in [SW|Jörg Stülke]'s lab

GP2010: expression of ''darA''-Strep in ''B. subtilis'' (chromosomal), suitable for [SW|SPINE], available in [SW|Jörg Stülke]'s lab

References

Reviews

25869574

25869574, 32095817, 32472931, 32603625

labs

[SW|Jörg Stülke], University of Göttingen, Germany [http://genmibio.uni-goettingen.de Homepage]